Academic degree
BDS, MHPE, PhD

Research interest
am leading two research groups at Khyber Medical University Pakistan and Zarqa University Jordan. My research interests include role of microbiome (oral and gut microbiome) in human health, diseases (Oral cancer, Tuberculosis) and conditions (malnutrition such stunting, wasting, overweight and obesity). My research also focusses on impact of dietary intake and tobacco use on oral microbiome and health.

Publications 

1. Shahzad M*, Saeed M, Amin H, Binmadi N, Ullah Z, Bibi S, Andrew SC*. The oral microbiome of newly diagnosed tuberculosis patients; a pilot study. Genomics. 2024 Feb 29;116(2):110816. doi: 10.1016/j.ygeno.2024.110816. Epub ahead of print. PMID: 38431030. 2. Saeed, M., Asad, M., Shahzad, M*., Akram, M., Khan, Z., 2024. Biochemical composition of a smokeless tobacco product (NASWAR) used in Pakistan. Journal of King Saud University - Science 36, 103168. https://doi.org/10.1016/j.jksus.2024.103168 3. Iddrisu I, Monteagudo-Mera A, Poveda C, Shahzad M, Walton GE, Andrews SC.
2. A review of the effect of iron supplementation on the gut microbiota of children in developing countries and the impact of prebiotics. Nutrition Research Reviews. Published online 2024:1-9. doi:10.1017/S0954422424000118 4.
3. Ahmad I, Binmadi N, Afridi SG, Aljohani S, Shah I, Saidal A, Shahzad M*. Salivary Oxidative Stress and Antioxidant Capacity in Smokeless Tobacco (Naswar) Users. Clin Cosmet Investig Dent. 2023;15:121-132 https://doi.org/10.2147/CCIDE.S415827 5. Binmadi, N.; Alsharif, M.; Almazrooa, S.; Aljohani, S.; Akeel, S.; Osailan, S.; Shahzad, M.; Elias, W.; Mair, Y. Perineural Invasion Is a Significant Prognostic Factor in Oral Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis. Diagnostics 2023, 13, 3339. https://doi.org/10.3390/diagnostics13213339 6.
4. Z Kamal, M Shahzad, TA Khan, F Niaz, K Hayat, Oral Candidal Carriage and its Correlation with Salivary Oxidative Stress in Smokeless Tobacco Users, Pakistan Journal of Medical & Health Sciences 17 (05), 534-534 7. Ullah H, Aziz T, Sarwar A, Khan Z, Shahzad M*. Alharbi M Alsahammari A. Evaluating the bacterial diversity of smokeless tobacco product using shotgun metagenomic analysis. Applied Ecology and Environmental Research 2023. 21(4):3045-3056. 8. Khan, K.; Qadir, A.; Trakman, G.; Aziz, T.; Khattak, M.I.; Nabi, G.; Alharbi, M.; Alshammari, A.; Shahzad, M*. Sports and Energy Drink Consumption, Oral Health Problems and Performance Impact among Elite Athletes. Nutrients 2022, 14, 5089. https://doi.org/10.3390/nu14235089 9. Aziz T, Xingyu H, Sarwar A, Naveed M, Shabbir MA, Khan AA, Ulhaq T, Shahzad M, Zhennai Y, Shami A, Sameeh MY, Alshareef SA, Tashkandi MA and Jalal RS (2023) Assessing the probiotic potential, antioxidant, and antibacterial activities of oat and soy milk fermented with Lactiplantibacillus plantarum strains isolated from Tibetan Kefir. Front. Microbiol. 14:1265188. doi: 10.3389/fmicb.2023.1265188 10. Article Citation: Raees M, Jamil B, Khan S, Shahzad M. Role of supervisor as clinical teacher – residents’ perspectives of postgraduate medical institute. Professional Med J 2023; 30(09):1208-1213. https://doi.org/10.29309/TPMJ/2023.30.09.5466 11. Ling L, Liu Y, Zhang X, Aziz T, Shahzad M, Sameeh MY, Wang Y, Cai C and Zhu Y (2023) Effect of Flammulina velutipes polysaccharides on the physicochemical properties of catfish surimi and myofibrillar protein oxidation during frozen storage. Front. Nutr. 10:1268580. doi: 10.3389/fnut.2023.126858 12. Aziz T, Naveed M, Makhdoom SI, Ali U, Mughal MS, Sarwar A, Khan AA, Zhennai Y, Sameeh MY, Dablool AS, Alharbi AA, Shahzad M, Alamri AS, Alhomrani M. Genome Investigation and Functional Annotation of Lactiplantibacillus plantarum YW11 Revealing Streptin and Ruminococcin-A as Potent Nutritive Bacteriocins against Gut Symbiotic Pathogens. Molecules. 2023; 28(2):491. https://doi.org/10.3390/molecules28020491 13. Shahzad M*, Andrews SC, Ul-Haq Z. Exploring the role of Microbiome in Susceptibility, Treatment Response and Outcome among Tuberculosis Patients from Pakistan: study protocol for a prospective cohort study (Micro-STOP) BMJ Open 2022;12:e058463. doi: 10.1136/bmjopen-2021-058463 14. Wang J, Aziz T, Bai R, Zhang X, Shahzad M, Sameeh MY, et al. Dynamic change of bacterial diversity, metabolic pathways, and flavor during ripening of the Chinese fermented sausage. Front Microbiol. 2022 ;13. Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2022.990606 15. Khan, M.S.; Saeedullah, A.; Andrews, S.C.; Iqbal, K.; Qadir, S.A.; Shahzad, B.; Ahmed, Z.; Shahzad, M*. Adolescent Afghan Refugees Display a High Prevalence of Hyperhomocysteinemia and Associated Micronutrients Deficiencies Indicating an Enhanced Risk of Cardiovascular Disease in Later Life. Nutrients 2022, 14, 1751. https://doi.org/10.3390/nu14091751. 16. Shah, S.; Khan, A.; Shahzad, M.; Mokhtar, J.A.; Harakeh, S.; Kibria, Z.; Mehr, A.; Bano, B.; Ali, A.; Yousafzai, Y.M. Determinants of Response at 2 Months of Treatment in a Cohort of Pakistani Patients with Pulmonary Tuberculosis. Antibiotics 2022, 11, 1307. https://doi.org/10.3390/antibiotics11101307 17. Saleem A, Afzal M, Naveed M, Makhdoom SI, Mazhar M, Aziz T, Khan AA, Kamal Z, Shahzad M, Alharbi M, Alshammari A. HPLC, FTIR and GC-MS Analyses of Thymus vulgaris Phytochemicals Executing In Vitro and In Vivo Biological Activities and Effects on COX-1, COX-2 and Gastric Cancer Genes Computationally. Molecules. 2022; 27(23):8512. https://doi.org/10.3390/molecules27238512 18. S ur Rahman, M Zahid, AA Khan, T Aziz, Z Iqbal, W Ali, FF Khan, S Jamil, Shahzad M, Mehtab A, AbdulRehman A. Hepatoprotective effects of walnut oil and Caralluma tuberculata against paracetamol in experimentally induced liver toxicity in mice, Acta Biochimica Polonica 69 (4), 871-878 19. Hena Zahid, Mohsin Shahab, Shafiq ur Rahman, Zafar Iqbal, Ayaz Ali Khan, Tariq Aziz, Waqar Ali, Ghazala Yasmin Zamani, Saeed Ahmad, Muhammad Shahzad, Metab Alharbi, Abdulrahman Alshammari. Assessing the effect of walnut (juglans regia) and olive (olea europaea) oil against the bacterial strains found in Gut Microbiome. Progress in Nutrition 2022; Vol. 24, N. 3: e2022122 DOI: 10.23751/pn.v24i3.13311 20. Naveed, M.; Shabbir, M.A.; Ain, N.-u.; Javed, K.; Mahmood, S.; Aziz, T.; Khan, A.A.; Nabi, G.; Shahzad, M.; Alharbi, M.E.; Alharbi, M.; Alshammari, A. Chain-Engineering-Based De Novo Drug Design against MPXVgp169 Virulent Protein of Monkeypox Virus: A Molecular Modification Approach. Bioengineering 2023, 10, 11. https://doi.org/10.3390/bioengineering10010011 21. Aziz, T.; Naveed, M.; Sarwar, A.; Makhdoom, S.I.; Mughal, M.S.; Ali, U.; Yang, Z.; Shahzad, M.; Sameeh, M.Y.; Alruways, M.W.; Dablool, A.S.; Almalki, A.A.; Alamri, A.S.; Alhomrani, M. Functional Annotation of Lactiplantibacillus plantarum 13-3 as a Potential Starter Probiotic Involved in the Food Safety of Fermented Products. Molecules 2022, 27, 5399. https://doi.org/10.3390/molecules27175399 22. Naveed, M.; Sheraz, M.; Amin, A.; Waseem, M.; Aziz, T.; Khan, A.A.; Ghani, M.; Shahzad, M.; Alruways, M.W.; Dablool, A.S.; Elazzazy, A.M.; Almalki, A.A.; Alamri, A.S.; Alhomrani, M. Designing a Novel Peptide-Based Multi-Epitope Vaccine to Evoke a Robust Immune Response against Pathogenic Multidrug-Resistant Providencia heimbachae. Vaccines 2022, 10, 1300. https://doi.org/10.3390/vaccines10081300 23. Naveed, M.; Makhdoom, S.I.; Ali, U.; Jabeen, K.; Aziz, T.; Khan, A.A.; Jamil, S.; Shahzad, M.; Alharbi, M.; Alshammari, A. Immunoinformatics Approach to Design Multi-Epitope-Based Vaccine against Machupo Virus Taking Viral Nucleocapsid as a Potential Candidate. Vaccines 2022, 10, 1732. https://doi.org/10.3390/vaccines10101732 24. Naveed M, Mughal MS, Jabeen K, Aziz T, Naz S, Nazir N, Shahzad M, et al. Evaluation of the whole proteome to design a novel mRNA-based vaccine against multidrug-resistant Serratia marcescens. Front Microbiol. 2022;13. Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2022.960285 25. Naveed, M.; Bukhari, B.; Aziz, T.; Zaib, S.; Mansoor, M.A.; Khan, A.A.; Shahzad, M.; Dablool, A.S.; Alruways, M.W.; Almalki, A.A.; Alamri, A.S.; Alhomrani, M. Green Synthesis of Silver Nanoparticles Using the Plant Extract of Acer oblongifolium and Study of Its Antibacterial and Antiproliferative Activity via Mathematical Approaches. Molecules 2022, 27, 4226. https://doi.org/10.3390/molecules27134226 26. Muhammad Nisar Khan, Haleema Khan, Muhammad Shahzad, Muhammad Ibrahim, Muhammad Arif, Zeeshan Kibria, Usman Waheed, Noore Saba, Inayat Shah, Sumera, Yasar Mehmood Yousafzai: Seroprevalence of Sars-Cov-2 antibodies among eligible blood donors of Peshawar, Pakistan. Am J Blood Res 2022;12(3):88-96. 27. Tariq Aziz, Abid Sarwar, Zubaida Daudzai, Jasra Naseeb, Jalal Ud Din, Urooj Aftab, Ahsan Saidal, Mustajab Ghani , Ayaz Ali Khan, Sumaira Naz , Muhammad Shahzad, Haiyang Cui * Lin Lin A*. Conjugated fatty acids (cfas) production via various bacterial strains and their applications. A review. J. Chil. Chem. Soc., 67, N°1 (2022) 28. Aziz T, Sarwar A, Naveed M, Shahzad M, Aqib Shabbir M, Dablool AS, Ud Din J, Ali Khan A, Naz S, Cui H, Lin L. Bio-Molecular analysis of selected food derived Lactiplantibacillus strains for CLA production reveals possibly a complex mechanism. Food Res Int. 2022 Apr;154:111031. doi: 10.1016/j.foodres.2022.111031. Epub 2022 Feb 17. PMID: 35337595. 29. Adil Hussain, Ayaz Ali Khan, Muhammad Ali, Ghazala Yasmin Zamani, Zafar Iqbal, Qarib Ullah, Javid Iqbal, Muhammad Shahzad, Tariq Aziz*. In-vitro AND In-vivo Assessment of Toxic effects of Parthenium hysterophorus leaves extract. J. Chil. Chem. Soc., 67, N°2 (2022). 30. Ahmad HA, Khan H, Shahzad M, Haq Z ul, Harake S, Yousafzai YM. Suspected reinfections of SARS-COV-2 in Khyber Pakhtunkhwa, Pakistan – analysis of province-wide testing database. J Infect. Available from: https://doi.org/10.1016/j.jinf.2021.10.005 31. H Ullah, M Shahzad*, F Saleem, T Ali, MK Azim, H Khan, J Ali, J Ahmed. Microbial Biofilm Diversity and Prevalence of Antibiotic Resistance Genes in Drinking Water Distribution System of Peshawar, Pakistan. Water 2021 13 (13), 1788 32. M Shahzad, A Asif, S Inayat, A Kinza, J Brekhna, S Abdul. An observational study to assess satisfaction with multi-resource peer assisted learning among postgraduate research students. Rawal Medical Journal 46 (2021) (3), 690 – 694 33. Iddrisu, I.; Monteagudo-Mera, A.; Poveda, C.; Pyle, S.; Shahzad, M.; Andrews, S.; Walton, G.E. Malnutrition and Gut Microbiota in Children. Nutrients 2021, 13, 2727. https://doi.org/10.3390/nu13082727 34. Saeedullah A, Khan MS, Andrews SC, Iqbal K, Ul-Haq Z, Qadir SA, Haris Khan, Ishawu Iddrisu, Muhammad Shahzad* . Nutritional Status of Adolescent Afghan Refugees Living in Peshawar, Pakistan. Nutrients 2021;13(9). https://doi.org/10.3390/nu13093072 35. Shahzad B, Shahzad M*, Khan MJ, Khan S, Bibi H. Plasma vitamin D status and associated factors among pregnant women of Peshawar, Khyber Pakhtunkhwa, Pakistan: a pilot study. Khyber Med Univ J 2019;11(4):226-30. DOI: 10.35845/kmuj.2019.19274 36. Hakim M, Khattak FA, Muhammad S, Ismail M, Ullah N, Atiq Orakzai M, Ulislam S, Ul-Haq Z. Access and Use Experience of Personal Protective Equipment Among Frontline Healthcare Workers in Pakistan During the COVID-19 Emergency: A Cross-Sectional Study. Health Secur. 2020 Nov 9. doi: 10.1089/hs.2020.0142. Epub ahead of print. PMID: 33175583 37. Inayat Shah, Saman Tauqir, Waheed Mughal, Muhamamd Shahzad, Tasleem Arif. Effective intensity of exercise for improving the modifiable risk factors of cvd in overweight adult males. The Spark. Volume 5, Issue 1, 2020. 38. Farhad Ali Khattak, Khalid Rehman, Muhammad Shahzad, Numan Arif, Naeem Ullah, Zeeshan Kibria, Muhammad Arshad, Saima Afaq, Arsalan Khan Ibrahimzai, Zia ul Haq. Prevalence of Parental refusal rate and its associated factors in routine immunization by using WHO Vaccine Hesitancy tool: A Cross sectional study at district Bannu, KP, Pakistan. International journal of infectious disease, 2020: DOI:https://doi.org/10.1016/j.ijid.2020.12.029. 39. Zia Ul-Haq, Shahzad M, Maria Ishaq Ktk et al. Clinical Characteristic, Mortality and Associated risk factor in COVID-19 patients reported in ten major hospitals of from Khyber Pakhtunkhwa, Pakistan. Journal of Ayub Medical College. Vol 32, No 4-Sup (2020). 40. Syed Abdul Qadir , Shahzad Muhammad* , Maria Ishaq Khattak , Zohaib Khan , Muslim Khan , Zia Ul Haq. Self-reported oral health status and associated factors among Afghan refugees in Peshawar Pakistan; a pilot study. Rehman Journal of Health Sciences, Vol 2, No1, 2020 41. Ma ZF, Ahmad J, Zhang H, Khan I, Muhammad S. Evaluation of phytochemical and medicinal properties of Moringa (Moringa oleifera) as a potential functional food. South African Journal of Botany. 2019. https://doi.org/10.1016/j.sajb.2018.12.002. 42. Khan S, Shahzad M*, Khan MJ, Shahzad B. Assessment of plasma iron and zinc status and its relationship with dietary intake and body mass index in antenatal women. RMJ. 2019; 44(3): 457-460. 43. Qadir SA, Shahzad M, Yousafzai YM. Assessment of oral health related knowledge, attitude, and self-reported practices of families residing in Peshawar, PakistanAdv Basic Med Sci. 2019;3(1): 54-58 44. Ravichanthiran K, Ma ZF, Zhang H, Cao Y, Wang CW, Muhammad S, Aglago EK, Zhang Y, Jin Y, Pan B. Phytochemical Profile of Brown Rice and Its Nutrigenomic Implications. Antioxidants 2018, 7(6), 71; https://doi.org/10.3390/antiox7060071. 45. Ayesha Syed, Falak Zeb, Saleem Khan, Mahpara Safdar, Iftikhar Alam, Muhammad Shahzad, Fazli Akbar, Feng Qing, Xiaoyue Wu and Masooma MunirExploration of Eating Disorders, Nutritional and Health Status Based on Eating Attitude Test-26 amongst University Adolescent Girls. Journal of Food and Nutrition Research. 2018, 6(7), 464-470. DOI: 10.12691/jfnr-6-7-7. 46. Shahzad, M., Sherry, L., Rajendran, R., Edwards, C. A., Combet, E. & Ramage, G. 2014. Utilising polyphenols for the clinical management of Candida albicans biofilms. International Journal of Antimicrobial Agents, 44, 269-273. 47. Shahzad, M., Millhouse, E., Culshaw, S, Edwards, C. A., Ramage, G & Combet, E. 2015. Selected dietary (poly)phenols inhibit periodontal pathogen growth and biofilm formation. Food & Function, 2015 Mar 11;6(3):719-29. doi: 10.1039/c4fo01087f. 48. Alalwan H, Rajendran R, Lappin DF, Combet E, Shahzad M, Robertson D, Nile CJ, Williams C & Ramage G (2017). The anti-adhesive effect of curcumin on Candida albicans biofilms on denture materials. Front. Microbiol. 8:659. 49. Shahzad, M, G. Ramage., C. A. Edwards And E. Combet 2013. Evaluation of the antibacterial potential of polyphenols against periodontal pathogens. Proceedings of the Nutrition Society, Volume 72 / Issue OCE4 / January 2013 (IF: 6.297). (Abstract) 50. Shahzad, M., Sherry, L., Rajendran, R., Edwards, C. A., Combet, E. & Ramage, G. 2014. Inhibitory effect of plant (poly)phenolics on growth and biofilm formation by Candida albicans. Proceedings of the Nutrition Society, Volume 73/ Issue OCE1/2014 (Abstract)

Currently serving as a lab supervisor at Zarqaa University, holding a master's degree in Biology from Hashemite University, specializing in cytotoxicity and genotoxicity. Her research interests center around cell culture techniques and molecular assays, with a focus on unraveling the intricate mechanisms of biological processes. Miss Yasmin contributed to the scientific community through her publication in The Natural Products Journal.

Employee at Zarqa University, holding Master's degree in Microbiology and Bachelor's degree in Biology from Hashemite University. Ms. Esraa is working as lab supervisor at the faculty of dentistry, she has papers published in scientific journals, and her research interests are in Molecular Microbiology and Medical analysis.